Recombinant Human FGF2

货号：913-FG2

规格：1mg

100ug

品牌：OrganRegen

产品介绍

Background:

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the FGF superfamily of mitogenic proteins which show 35-60% amino acid conservation[1]. FGF acidic and basic are unique from other members of the family in that they lack classical secretory signal peptides. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury[2]. FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may also participate in the development of several pathological conditions resulting from excessive cell proliferation and/or angiogenesis[3]. BFGF is a critical component of human embryonic stem cell culture medium[4].

技术参数

Source:

E.coli

Protein Construction:

A DNA sequence encoding the amino acids (Pro143-Ser288) of human FGF-2 (Accession Number:

P48798) was expressed.

Synonyms:

Basic fibroblast growth factor; bFGF; FGF basic; FGF-2.

SPECIFICATIONS

Purity:

≥ 97%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 10-50 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

16.5 kDa

SDS-PAGE:

18 kDa, reducing conditions

DATA

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

References:

1.          Takayama, S., et al., Periodontal regeneration by FGF-2 (bFGF) in primate models. Journal of dental research, 2001. 80(12): p. 2075-9.

2.          Penaultllorca, F., et al., Expression of variant forms of fibroblast growth factor receptor 2 mRNA in human breast carcinomas. International journal of oncology, 1997. 10(6): p. 1191-6.

3.          Niu, Y., et al., Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chinese medical journal, 2004. 117(2): p. 252-7.

4.          Zhang, Y., et al., Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chinese medical journal, 2004. 117(4): p. 601-3.

‍Recombinant Human FGF7

货号：923-FG7

规格：1mg

100ug

品牌：OrganRegen‍

产品介绍

DESCRIPTION

Background:

FGF-7 (fibroblast growth factor-7) is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis[1].FGF-7 promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation[2]. It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies. Deletion of FGF-7 affects kidney development, producing abnormally small ureteric buds and fewer nephrons[3] . It also impedes hair follicle differentiation [4]. The 194 amino acid (aa) FGF-7 precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites.

技术参数

Source:

Chinese Hamster Ovary cell line

Protein Construction:

A DNA sequence encoding the amino acids (Cys32-Thr194) of human FGF-7 (Accession Number:

P21781) was expressed.

Synonyms:

HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF.

SPECIFICATIONS

Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 5-10 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

19 kDa

SDS-PAGE:

22 kDa, reducing conditions 

DATA

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

References:

1.          Finch, P.W. and J.S. Rubin, Keratinocyte growth factor expression and activity in cancer: implications for use in patients with solid tumors. J Natl Cancer Inst, 2006. 98(12)

2.          Niu, J., et al., Keratinocyte growth factor/fibroblast growth factor-7-regulated cell migration and invasion through activation of NF-kappaB transcription factors. J Biol Chem, 2007. 282(9)

3.          Qiao, J., et al., FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development, 1999. 126(3)

4.          Guo, L., L. Degenstein, and E. Fuchs, Keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev, 1996. 10(2):