Sephadex G-25 in PD-10 Desalting Columns
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
- Flexible run protocols – perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
- Flexible throughput – desalt one sample or multiple samples in parallel
- Good recovery – in the range of 70% to > 95%
- High desalting capacity – remove > 98% salt with gravity; > 90% with centrifugation
- Minimal dilution – typically none when used as spin columns; 1.4× when used with gravity flow
- Time savings compared to dialysis – inexpensive and convenient alternative to lengthy and troublesome dialysis procedures
What comes in the PD-10 column package?
Each product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.
Versatile for use with centrifugation or gravity flow
The product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Successful use of these prepacked desalting columns
The following publications demonstrate the utility of PD-10 columns:
- desalting of membrane proteins after anion exchange chromatography
- desalting of variants of an essential reductase involved in fatty acid biosynthesis
- buffer exchange of an affinity-tagged acyl carrier protein
Accessories for PD-10 Desalting Columns
A LabMate buffer reservoir can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.
Other Sephadex G-25 columns from GE
GE Healthcare’s Life Sciences solutions for desalting include a wide range of columns containing Sephadex G-25 resin. Options are available to accommodate sample volumes from 100 µL to 15 mL and use with manual methods or a chromatography system. Let GE help you find the right desalting product.
GE 17-0851-01 Disposable PD-10脱盐层析柱的详细介绍
GE 17-0851-01 Disposable PD-10脱盐层析柱PD-10脱盐层析柱与一个方便的直立层析柱架一起提供。使用LabMate缓冲液储液器使柱平衡过程更容易进行。
• 快速、高回收率的脱盐和缓冲液置换，也可用于除小分子化合物；预装Sephadex G-25填料。
• 操作灵活，可用离心或重力流。
• 容易使用，便于平衡、上样、洗脱。
• 重力流方式样品量1-2.5ml；离心方式样品量1.75-2.5ml。平行处理多个待脱盐样品，可除去90%以上盐离子。
• 蛋白回收率在70%-95%。
• LabMate Buffer Reservoir使得平衡PD-10层析柱更容易，更方便。
PD-10层析柱提供了一个经济、方便的选择，可取代冗长、繁琐的透析过程。几分钟内，完成生物样品脱盐、小分子可溶物的去除或替换，同时有极好的回收率。
Disposable PD-10 Desalting Columns经过改进设计，能应用于两种新操作方式：重力流和离心，更为灵活方便。重力流操作可选用一到数根柱子，平行操作多个样品进行除杂处理，而无需纯化设备。离心操作则能以标准的离心程序平行处理洗脱样品，将样品稀释降低到最少。
每个包装内含有四个接头，方便连接配置。与LabMate Buffer Reservoir（需另行购买）联合使用进行重力流操作时，清洗和平衡用的缓冲液能在同一步中加入。
如需要设计经济适用，通用性好的制备样品工艺，推荐购买PD-10 columns和批量包装的填料。
Disposable PD-10脱盐预装层析柱每盒有30根。每根柱均保存在含有0.15% Kathon CG保护剂的蒸馏水中。颗粒大小为20-85μm的填料填充在两个烧结的聚乙烯熔块中。熔块保护填料免于在重力缓冲液流下而干柱。层析柱的出口用可重复使用的帽封口。
重力流操作，从BSA中除去NaCl。样品回收率95%，除盐99%以上。
PD-10 Desalting Columns能以重力流或离心方式快速脱盐。蓝色接头用于离心操作
